Click Import to transfer the waypoints from the GPS to the computer. Under Import, leave only the Waypoint checkbox selected. Under Device, click on the radio buttons for the type of GPS used. Moreover, fluorescence microscopy was used to monitor the apoptotic cell death of gastric epithelial monolayers on-a-chip in response to camptothecin, a therapeutic gastric cancer drug. Step 3-Import Waypoints into Google Earth. The generation of a monolayer was faster than in the static Boyden chamber. The barrier integrity was assessed by the FITC-dextran test. Media flow at a constant rate (0.5 μL/min) rapidly led the gastric epithelial cells to develop into a polarized monolayer. The continuous flow rate was achieved using a pressure-driven pump. It displays satellite images, aerial photographs, and graphic layers on personal computers by delivering them over the Internet. We present considerations on how to ensure continuous and stable flow through the channels. The fully transparent microfluidic chip consists of two microfluidic channels separated by a collagen-coated porous membrane and lined by human polarized gastric epithelial (NCI-N87) cells. Therefore, this study focuses on establishing a polarized gastric epithelial monolayer on-a-chip and describes a general-purpose methodology applicable for bonding any porous material to PDMS through an adhesive sublayer. Several cell lines could be cultivated in microengineered environments with promising results, but gastric cell lines have not yet been widely used or studied.

Microfluidic devices for culturing cells have been successfully utilized for biomedical applications, including drug screening.

Google earth download chip online